That brings the number of Saints regulars sitting out Thursday night's nationally televised game to nine.
Payton had said earlier that he might rest Colston, who has soreness in his left knee which has limited him during training camp. Payton said Colston could play with the pain but there was no need for him to push through it in an exhibition game.
Vilma, who missed much of last season because of a right knee injury that required reconstructive surgery, has been practicing off and on and was not expected to play in the opening preseason game.
Bullocks practiced on Tuesday, but has been resting intermittently during camp as he recovers from offseason arthroscopic surgery on his right knee.
Young has mild arthritis in his left knee, causing it to flare up when overworked. He has practiced some in training camp but has rested during the past few days.
On Monday, Payton said he would sit out several other projected regulars who have been limited in practice as they attempt to come back from injuries, including running back Deuce McAllister, tight end Jeremy Shockey and cornerback Mike McKenzie.
Rookie cornerback Tracy Porter and receiver Devery Henderson, who both recently went out with pulled hamstrings, also won't play.
Copyright 2008 by The Associated Press