Polamalu's tackle occurred at the end of Ricky Williams' 26-yard run late in the third quarter.
"It definitely wasn't on purpose," Polamalu said Wednesday. "I've never intentionally tried to horse-collar anybody."
It wasn't known if Polamalu would appeal the fine, and he didn't want to discuss it.
"I'm not a big fan of people being really vocal about the fines," he said.