Lee, the team leader in tackles, interceptions and fumble recoveries, dislocated his left wrist during last week's loss to the Philadelphia Eagles. He didn't practice all week and had been listed as doubtful.
The Cowboys also will be missing Pro Bowl punter Mat McBriar, who's dealing with a nerve issue in his left foot. McBriar was unable to finish last week's game because of the issue and had been listed as questionable. Chris Jones, added to the active roster off the practice squad Saturday, will handle punting duties.
The Associated Press contributed to this report.