The move announced Wednesday completes the coaching staff for next season. Holt takes over for Mike Tice, who was promoted to offensive coordinator after the departure of Mike Martz.
Tice was widely credited with getting the most out of an undermanned line the past two years and still figures to play a big role with that unit.
Holt spent the past three years on Tampa Bay's staff, serving as the assistant offensive line coach last season and as an offensive assistant in 2010. He joined the Buccaneers as a coaching intern in 2009.
He also coached for 14 years on the college level.