Polamalu, who has missed the Steelers' last two games, aggravated the injury during practice on Wednesday and has been unable to practice since.
Holmes, also out for two games, continues to have pain in the ankle and was urged earlier this week to sit out another game. Holmes said the game against New England (12-0) is too important to the Steelers (9-3) to miss and he plans to play.
Holmes has averaged 17 yards per reception on 39 catches, and leads the Steelers with seven touchdown catches.
Copyright 2007 by The Associated Press