Roethlisberger's attorney, Ed Garland, offered to provide a DNA sample from the quarterback, Georgia Bureau of Investigations officials confirmed to the Tribune-Review. However, they told Garland they didn't need it.
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Garland told The Associated Press on Tuesday night that officials withdrew the request a week ago. He declined to comment on why.
"Based on everything that I know and our own investigation, I believe that no charges should be filed in this case," Garland told the Tribune-Review.
A 20-year-old female college student has accused Roethlisberger of sexually assaulting her in a Milledgeville, Ga., nightclub in the early hours of March 5.
Roethlisberger also is facing a lawsuit in Nevada after a woman accused him of raping her in 2008 at a Lake Tahoe hotel. Roethlisberger also denies that allegation and has sued for counter-damages.
Roethlisberger hasn't been charged in either case, although the most recent accusation is still under investigation by the GBI.
According to the Pittsburgh Post-Gazette, NFL Commissioner Roger Goodell said Wednesday that he still intends to meet with Roethlisberger despite the fact Georgia investigators no longer want a DNA sample from the quarterback.
"We're following it," Goodell said. "It's not something I've put aside. I'm focusing on it."
Team president Art Rooney has said the Steelers are "in a situation we're going to let this investigation play out and then go from there."
"My concerns are many, but I think at this time it's kind of appropriate to watch these things and let these things run their course," Tomlin said Saturday. "I think it would be inappropriate for me to have strong feelings one way or another with the investigation being ongoing and so forth. Like everyone else, you watch these things unfold."
The Associated Press contributed to this report.