The Cowboys said Bryant injured his ankle during practice Friday. He already had been limited in practice this week because of the back issue.
Bryant hasn't missed any games despite having a variety of ankle, back and rib injuries throughout the season. He leads the Cowboys with six touchdowns and ranks third in catches (44) and receiving yards (554).
Linebackers Keith Brooking and Bradie James also were held out of practice Friday. Brooking is questionable with a foot problem. James is probable with a knee injury.
The Associated Press contributed to this report.