Best, the Lions' leading rusher (382 yards) and third-leading receiver (50 receptions), has battled turf toe injuries all season.
Best was limited to 2 yards on three carries and four catches for 15 yards Sunday against the Dallas Cowboys. Lions coach Jim Schwartz said Monday that Best's health hasn't improved as the season has gone on, and there was some doubt as to whether or not the rookie could play this week.
Three-fourths of Detroit's starting defensive line also appears on the injury report. Tackle Corey Williams (shoulder) and end Kyle Vanden Bosch (knee) didn't practice, and end Cliff Avril (quadriceps) was limited. Williams and Avril are listed as questionable, but Vanden Bosch is probable.
Linebackers Isaiah Ekejiuba (knee) and center Dylan Gandy (calf) were limited at practice Wednesday and also are listed as questionable.
The Lions have declared quarterback Matthew Stafford, defensive end Lawrence Jackson and kicker Jason Hanson out. Stafford has missed all but three games this season because of two different shoulder injuries, Hanson has missed the past two games with a knee injury, and Jackson sustained a concussion in the game against the Cowboys.
The Associated Press contributed to this report.