The 31-year-old bruising running back announced his retirement from the NFL on Thursday via his official Twitter account.
"After 9 years in this whirlwind business they call the NFL, I am proud to announce that I am hanging up my cleats. I've had an amazing run and I appreciate all of the support from the fans through the good and the bad.
"The New York Giants are a great organization and I am proud to have been a part of it for so long," Jacobs went on. "I love my coaches and my teammates and will miss them as well as the game itself but I am healing well from my surgery and I look forward to running around with my kids for years to come."
Jacobs retires first on the Giants' all-time rushing touchdowns list (60) and fourth on the career rushing yards list (5,087). He spent the first eight years of his professional career on the Giants, the team that drafted him in the fourth round of the 2005 draft, before returning for one final run in 2013.
Jacobs signed a one-year contract with the San Francisco 49ers prior to the 2012 season, but his tenure with the team was marred by controversy. He played sparingly with the Niners, appearing in just two of the team's first 13 regular-season games. Jacobs aired his grievances over lack of playing time on Twitter and was suspended for conduct detrimental to the team for the final three games. Jacobs said then that he would only play for the Giants, and his wish came true in 2013.
Jacobs picked up four touchdowns this season in seven games played (with one start). He rushed for 238 yards before he was placed on injured reserve with a knee injury in December.
The 264-pound Jacobs was abnormally tall for a running back at 6-foot-4, and despite his high center of gravity, was one of the best short-yardage running backs during his prime.
Jacobs will be best remembered for his pulverizing runs and for being next to impossible to tackle at his peak.