The NFL acted after Wilfork received a 15-yard penalty for grabbing the face mask of Chargers running back Michael Turner with his right hand during New England's 21-12 win over San Diego on Jan. 20.
The fine brings Wilfork's total to $37,500 this season.
Wilfork, a first-round draft choice from Miami in 2004, was fined $12,500 for a low, late hit on quarterback J.P. Losman on Buffalo's first series of the third game. Losman missed the rest of that game and the next two with a sprained left knee.
A $5,000 fine was imposed after the sixth game, when Wilfork was penalized for unsportsmanlike conduct for a late hit on Dallas tight end Jason Witten.
Wilfork was fined $15,000 after the regular-season finale, when he poked his finger inside the face mask of New York Giants running back Brandon Jacobs.
Copyright 2007 by The Associated Press